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nebnext quick ligation module  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext quick ligation module
    Nebnext Quick Ligation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+quick+ligation+module/NEBNext+Quick+Ligation+Module/pmc13049905-118-32-36
    Average 99 stars, based on 1073 article reviews
    nebnext quick ligation module - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Phage-assisted evolution of allosteric protein switches
    Article Snippet: End repair and A-tailing were performed using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, cat. no. E7546S), followed by barcode adapter ligation with the NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367L). .. Barcoded samples were pooled, purified with AMPure XP beads, and ligated to sequencing adapters using the NEBNext Quick Ligation Module (New England Biolabs, cat. no. E6056S). ..

    Article Title: Dominance modifiers at the Arabidopsis self-incompatibility locus retain proto-miRNA features and act through non-canonical pathways
    Article Snippet: Barcodes provided by ONT were then ligated using the Blunt/TA Ligase Master Mix (NEB). .. Barcoded fragments were purified with AMPure XP beads (Beckmann Coulter), then pooled and ONT adapters were added using the NEBNext Quick Ligation Module (NEB). .. After purification with AMPure XP beads (Beckmann Coulter), each library was mixed with the sequencing buffer (ONT) and the loading beads (ONT) and loaded on a PromethION R9.4.1 flow cell.

    Article Title: Containment of a Multi-index B.1.1.7 Outbreak on a University Campus Through a Genomically Informed Public Health Response
    Article Snippet: .. Oxford Nanopore sequencing adapters were ligated with the NEBNext Quick Ligation Module (NEB), and the library was purified with 1× volume of AMPure beads. .. Final libraries were quantified with the Qubit 1× dsDNA HS Assay Kit (Thermo Fisher).

    Sequencing:

    Article Title: Phage-assisted evolution of allosteric protein switches
    Article Snippet: End repair and A-tailing were performed using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, cat. no. E7546S), followed by barcode adapter ligation with the NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367L). .. Barcoded samples were pooled, purified with AMPure XP beads, and ligated to sequencing adapters using the NEBNext Quick Ligation Module (New England Biolabs, cat. no. E6056S). ..

    Article Title: Occurrence and subtyping of the intestinal protozoan Blastocystis sp. in French oyster areas reveal potential fecal contamination routes and associated risks to consumer health
    Article Snippet: Briefly, sequencing libraries were prepared with the Native Barcoding Kit (Oxford Nanopore Technologies) following the manufacturer's instructions. .. Amplicons were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, MA, USA), barcoded with the Blunt/TA Ligase Master Mix (NEB), and ligated to sequencing adapters using the NEBNext Quick Ligation Module (NEB). ..

    Article Title: Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting.
    Article Snippet: .. The library was prepared using Ligation Sequencing kit (SQK-LSK114; ONT, Oxford, UK), NEBNext®Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7180S) and NEBNext® Quick Ligation Module (NEB, E6056S) in accordancewith themanufacturer’s instructions.Theprepared librarywas loaded into the Flow Cell (R10.4.1) and run with PromethION for 72 h. Basecalling and barcode demultiplexing were performed by dorado (version 1.3.0). ..

    Ligation:

    Article Title: Phage-assisted evolution of allosteric protein switches
    Article Snippet: End repair and A-tailing were performed using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, cat. no. E7546S), followed by barcode adapter ligation with the NEB Blunt/TA Ligase Master Mix (New England Biolabs, cat. no. M0367L). .. Barcoded samples were pooled, purified with AMPure XP beads, and ligated to sequencing adapters using the NEBNext Quick Ligation Module (New England Biolabs, cat. no. E6056S). ..

    Article Title: Occurrence and subtyping of the intestinal protozoan Blastocystis sp. in French oyster areas reveal potential fecal contamination routes and associated risks to consumer health
    Article Snippet: Briefly, sequencing libraries were prepared with the Native Barcoding Kit (Oxford Nanopore Technologies) following the manufacturer's instructions. .. Amplicons were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, MA, USA), barcoded with the Blunt/TA Ligase Master Mix (NEB), and ligated to sequencing adapters using the NEBNext Quick Ligation Module (NEB). ..

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing
    Article Snippet: NEB Blunt/TA Ligase Master Mix , New England Biolabs , Cat# M0367L. .. T4 DNA ligase, from NEBNext Quick Ligation Module , New England Biolabs , Cat# E6056S. .. EcoRI , New England Biolabs , Cat# R0101S.

    Article Title: Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolates
    Article Snippet: NEBNext® Ultra II End repair/dA-tailing Module , New England BioLabs , E7546S. .. NEBNext® Quick Ligation Module , New England BioLabs , E6056S. .. Oxford Nanopore Technologies Ligation Sequencing Kit , Oxford Nanopore Technologies , SQK-LSK109.

    Article Title: Dominance modifiers at the Arabidopsis self-incompatibility locus retain proto-miRNA features and act through non-canonical pathways
    Article Snippet: Barcodes provided by ONT were then ligated using the Blunt/TA Ligase Master Mix (NEB). .. Barcoded fragments were purified with AMPure XP beads (Beckmann Coulter), then pooled and ONT adapters were added using the NEBNext Quick Ligation Module (NEB). .. After purification with AMPure XP beads (Beckmann Coulter), each library was mixed with the sequencing buffer (ONT) and the loading beads (ONT) and loaded on a PromethION R9.4.1 flow cell.

    Article Title: Containment of a Multi-index B.1.1.7 Outbreak on a University Campus Through a Genomically Informed Public Health Response
    Article Snippet: .. Oxford Nanopore sequencing adapters were ligated with the NEBNext Quick Ligation Module (NEB), and the library was purified with 1× volume of AMPure beads. .. Final libraries were quantified with the Qubit 1× dsDNA HS Assay Kit (Thermo Fisher).

    Article Title: Profiling of genetic determinants required for fitness of community-associated methicillin-resistant Staphylococcus aureus in human blood.
    Article Snippet: The fragmented DNA was subsequently processed to generate blunt-ended fragments using the NEBNext End Repair Module (New England Biolabs, Ipswich, MA, USA), followed by dA-tailing with the NEBNext dA-Tailing Module (New England Biolabs). .. Subsequently, the dA-tailed DNA was ligated Month XXXX Volume 0 Issue 0 10.1128/spectrum.03585-2514 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 24 A pr il 20 26 b y 20 01 :4 48 a: 20 02 :7 09 2: 61 0e :1 86 9: ac 34 :d dd 1. to SpIA5 adapters using the NEBNext Quick Ligation Module. ..

    Article Title: Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targeting.
    Article Snippet: .. The library was prepared using Ligation Sequencing kit (SQK-LSK114; ONT, Oxford, UK), NEBNext®Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7180S) and NEBNext® Quick Ligation Module (NEB, E6056S) in accordancewith themanufacturer’s instructions.Theprepared librarywas loaded into the Flow Cell (R10.4.1) and run with PromethION for 72 h. Basecalling and barcode demultiplexing were performed by dorado (version 1.3.0). ..



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